Human IRF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4830
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IRF1
Thr147-Pro325
Accession # P10914
Thr147-Pro325
Accession # P10914
Specificity
Detects human IRF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IRF1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IRF1 Antibody
Detection of Human IRF1 by Western Blot.
Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human IRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4830) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IRF1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.Applications for Human IRF1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Nalm‑6 human Pre-B acute lymphocytic leukemia cell line and Jurkat human acute T cell leukemia cell line
Sample: Nalm‑6 human Pre-B acute lymphocytic leukemia cell line and Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRF1
IRF1 (interferon regulatory factor 1) is a 45 kDa member of the IRF family of proteins. It is induced by TLR9 and INF-gamma stimulation, and positively regulates the expression of IFN-beta, iNOS and 1L-12p35. Human IRF1 is 325 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 1‑140) and an acidic C-terminus (aa 147‑325). Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11 aa substitution for the C-terminal 86 amino acids. Over aa 171‑325, human IRF1 shares 83% aa identity with both canine and porcine IRF1.
Long Name
Interferon Regulatory Factor 1
Alternate Names
MAR
Gene Symbol
IRF1
UniProt
Additional IRF1 Products
Product Documents for Human IRF1 Antibody
Product Specific Notices for Human IRF1 Antibody
For research use only
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