Human JunB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4456
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 512313
Product Summary for Human JunB Antibody
Immunogen
E. coli-drived recombinant human JunB
Asp145-Val246 (predicted)
Accession # P17275
Asp145-Val246 (predicted)
Accession # P17275
Specificity
Detects human JunB in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) JunD or rhc-Jun is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human JunB Antibody
Detection of Human JunB by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human JunB Monoclonal Antibody (Catalog # MAB4456) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for JunB at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human JunB Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JunB
Long Name
JunB Proto-oncogene
Alternate Names
activator protein 1, AP-1, jun B proto-oncogene, transcription factor jun-B
Gene Symbol
JUNB
UniProt
Additional JunB Products
Product Documents for Human JunB Antibody
Product Specific Notices for Human JunB Antibody
For research use only
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