Human Kallikrein 15 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2540
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 15 isoform 1
Asp17-Asn255
Accession # AAI44047
Asp17-Asn255
Accession # AAI44047
Specificity
Detects human Kallikrein 15 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Kallikrein 2, rhKallikrein 8, and rhKLK11 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Kallikrein 15 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 15, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 15, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Kallikrein 15
Sample: Recombinant Human Kallikrein 15
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 15
Kallikrein 15, encoded by the KLK15 gene, is a member of the tissue Kallikrein family of Serine proteases. It is a secreted protein found in prostate, colon, thyroid tissues, breast milk and seminal plasma. Whether it has a trypsin-like activity or not remains controversial. Several splice forms exist. The 250 aa rhKLK15 has a 10‑His tag at the C-terminus. It predicts a molecular mass of 28 kDa and migrates as a 39 kDa band in SDS-PAGE under reducing conditions. The amino acid sequence of human KLK15 is 99%, 77% and 77% identical to that of chimpanzee, canine and mouse, respectively.
Additional Kallikrein 15 Products
Product Documents for Human Kallikrein 15 Antibody
Product Specific Notices for Human Kallikrein 15 Antibody
For research use only
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