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Human Kallikrein 3/PSA Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB13442

R&D Systems, part of Bio-Techne
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MAB13442-100
MAB13442-500
MAB13442-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair), Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 181811

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3/PSA
Pro19-Pro261
Accession # P07288

Specificity

Detects human Kallikrein 3/PSA in sandwich immunoassays and in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Kallikrein 3/PSA Antibody

Detection of Human Kallikrein 3/PSA antibody by Western Blot.

Detection of Human Kallikrein 3/PSA by Western Blot.

Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13442) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Kallikrein 3/PSA at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Kallikrein 3/PSA Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human prostate tissue

Human Kallikrein 3/PSA Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Kallikrein 3/PSA Biotinylated Antibody (Catalog # BAF1344)
  • Standard: Recombinant Human Kallikrein 3/PSA Protein, CF (Catalog # 1344-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 3/PSA

Kallikrein 3 (KLK-3) is a member of the Kallikrein family of serine proteases. It is also known as PSA (prostate specific antigen) and is used in prostate cancer diagnosis.

Alternate Names

KLK3, PSA

Entrez Gene IDs

354 (Human)

Gene Symbol

KLK3

UniProt

Additional Kallikrein 3/PSA Products

Product Documents for Human Kallikrein 3/PSA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Kallikrein 3/PSA Antibody

For research use only

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