HEK293 human embryonic kidney cell line transfected with either (A) human SLC12A7 or (B) human MRGPRF and eGFP was stained with Mouse Anti-Human KCC4/SLC12A7 Monoclonal Antibody (Catalog # MAB9030) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human KCC4/SLC12A7 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human SLC12A7

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KCC4/SLC12A7

Cation chloride cotransporters, including the potassium-chloride cotransporters (KCCs), are involved in the electroneutral movement of ions across the plasma membrane. SLC12A7 contains 12 membrane-spanning segments, 8 phosphorylation sites, 7 of which are in the C terminus, and 4 potential N-glycosylation sites. It is important for potassium recycling by siphoning potassium ions after their exit from outer hair cells into supporting Deiters cells, where potassium enters the gap junction pathway.

Long Name

Potassium/Chloride Transporter KCC4

Alternate Names

SLC12A7

Entrez Gene IDs

10723 (Human); 20499 (Mouse); 308069 (Rat)

Gene Symbol

SLC12A7

UniProt

Q9Y666 (Human)

Additional KCC4/SLC12A7 Products

Product Documents for Human KCC4/SLC12A7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KCC4/SLC12A7 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human KCC4/SLC12A7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9030

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all KCC4/SLC12A7 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human KCC4/SLC12A7 Antibody

Detection of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7 and eGFP by Flow Cytometry.