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Human Kremen-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2127

R&D Systems, part of Bio-Techne
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MAB2127
MAB2127-SP

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 301324

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kremen‑1
Ala20-Gly397
Accession # NP_114434

Specificity

Detects human Kremen-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse Kremen-1 or recombinant human Kremen-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Kremen-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Kremen-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kremen-1

Kremen-1 is a type I transmembrane protein that contains extracellular Kringle, WSC, and CUB domains. It is expressed in diverse embryonic and adult tissues. Kremen proteins bind the secreted Dickkopf proteins Dkk-1, -2, and -4 with high affinity to antagonize the canonical Wnt signaling pathway.

Long Name

Kringle-containing Transmembrane Protein 1

Alternate Names

Kremen1, KRM1

Entrez Gene IDs

83999 (Human); 84035 (Mouse)

Gene Symbol

KREMEN1

UniProt

Additional Kremen-1 Products

Product Documents for Human Kremen-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Kremen-1 Antibody

For research use only

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