Human Kremen-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2127
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kremen-1
Ala20-Gly397
Accession # NP_114434
Ala20-Gly397
Accession # NP_114434
Specificity
Detects human Kremen-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Kremen-1 and less than 5% cross-reactivity with recombinant human Kremen-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Kremen-1 Antibody
Kremen‑1 in Human Colon Cancer Tissue.
Kremen-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Kremen-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Kremen‑1 in Human Colon.
Kremen-1 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human Kremen-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Kremen-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013), and human colon array
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013), and human colon array
Western Blot
0.1 µg/mL
Sample: Recombinant Human Kremen-1
Sample: Recombinant Human Kremen-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kremen-1
Long Name
Kringle-containing Transmembrane Protein 1
Alternate Names
Kremen1, KRM1
Gene Symbol
KREMEN1
UniProt
Additional Kremen-1 Products
Product Documents for Human Kremen-1 Antibody
Product Specific Notices for Human Kremen-1 Antibody
For research use only
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