Human Lactase-like Protein/LCTL Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5984
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Lactase-like Protein/LCTL
Arg23-Glu541
Accession # Q6UWM7
Arg23-Glu541
Accession # Q6UWM7
Specificity
Detects human Lactase-like Protein/LCTL in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse Lactase-like Protein/LCTL is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Lactase-like Protein/LCTL Antibody
Detection of Human Lactase-like Protein/LCTL by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Lactase-like Protein/LCTL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5984) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lactase-like Protein/LCTL at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human Lactase-like Protein/LCTL Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Lactase-like Protein/LCTL, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Lactase-like Protein/LCTL, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line
Sample: A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lactase-like Protein/LCTL
LCTL (also Lactase-like Protein and Klotho/lactase-phlorizin hydrolase-related protein/KLPH) is a 62 kDa (predicted) member of the glycosyl hydrolase 1 family of proteins. It is expressed in skin and kidney, and may participate in endocrine FGF19 subfamily signaling pathways. Mature human LCTL is a 546 amino acid (aa) type I transmembrane protein that appears to be present in the ER. It contains an apparently nonfunctional glycosidase domain (aa 33‑503), plus a very short five aa cytoplasmic segment that may function as an ER-retention signal. There is one potential alternative start site at Met174. Over aa 23‑541, human LCTL shares 86% aa identity with mouse LCTL.
Alternate Names
KLG, KLPH, Lactase like Protein, LCTL
Gene Symbol
LCTL
UniProt
Additional Lactase-like Protein/LCTL Products
Product Documents for Human Lactase-like Protein/LCTL Antibody
Product Specific Notices for Human Lactase-like Protein/LCTL Antibody
For research use only
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