Detects human LAIR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LAIR2 or recombinant mouse LAIR1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human LAIR1 Antibody

Detection of LAIR1 in Human Lymphocytes by Flow Cytometry.

Human lymphocytes were stained with Mouse Anti-Human LAIR1 Monoclonal Antibody (Catalog # MAB2664, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LAIR1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human whole blood lymphocytes

Western Blot

1 µg/mL
Sample: Recombinant Human LAIR1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1; also CD305) is a 46 kDa inhibitory receptor belonging to the Ig superfamily. It is a type I transmembrane protein with one extracellular Ig-like domain and two cytoplasmic ITIMs. Four LAIR-1 splice variants exist. LAIR1b has a 17 aa deletion outside the Ig loop in the extracellular domain. It differs from LAIR1c by one aa residue. LAIR1d has a 77 aa truncation in the cytoplasmic domain. LAIR1 is expressed on NK cells, T cells, B cells, monocytes, dendritic cells and most thymocytes. The extracellular domain of human LAIR1 shares 40% aa identity with that of the mouse protein.

Long Name

Leukocyte-associated Ig-like Receptor 1

Alternate Names

CD305, HLAIR1, LAIR-1

Entrez Gene IDs

3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)

Gene Symbol

LAIR1

UniProt

Q6GTX8

Additional LAIR1 Products

Product Documents for Human LAIR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LAIR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LAIR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2664

Key Product Details

Species Reactivity

Validated:

Cited:

Applications