Human Laminin alpha1 N-Terminus Domain VI Antibody

Name: Human Laminin alpha1 N-Terminus Domain VI Antibody
Brand: R&D Systems
SKU: AF4187

R&D Systems, part of Bio-Techne | Catalog # AF4187

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Laminin alpha 1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Laminin alpha1 N-Terminus Domain VI
Leu22-Met269
Accession # P25391

Specificity

Detects human Laminin alpha1 N-Terminus Domain VI in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse LAMA4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Laminin alpha1 N-Terminus Domain VI Antibody

Detection of Laminin alpha 1 in U2OS Human Cell Line by Flow Cytometry.

U2OS human osteosarcoma cell line was stained with Goat Anti-Human Laminin alpha 1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF4187, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Laminin alpha1 N-Terminus Domain VI Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E10)

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: U2OS human osteosarcoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Laminin alpha1 N-Terminus Domain VI

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 1

Laminin subunit alpha1 (LAMA1) is a secreted 400 kDa extracellular matrix glycoprotein that contributes to the formation of basement membrane Laminin isoforms 1 and 3. It is one of three subunits ( alpha, beta, and gamma) that interact via their coiled-coil domains to form the approximately 800 kDa cruciform, disulfide-linked, Laminin heterotrimer. The 3058 amino acid (aa) residue mature human alpha1 chain contains an N-terminal Laminin VI domain (aa 18‑269), followed by domains V through III containing 17 EGF-like repeats, the coiled-coil domains II and I, and five globular, Laminin G-like domains. Over aa 22‑269, human Laminin alpha1 shares 95% and 91% aa sequence identity with canine and mouse alpha1 chain, respectively.

Alternate Names

LAMA1

Entrez Gene IDs

284217 (Human); 16772 (Mouse); 316758 (Rat)

Gene Symbol