Human LILRB4/CD85k/ILT3 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2425
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Western Blot
Cited:
ELISA Development (Detection), Flow Cytometry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRB4/CD85k/ILT3
Pro17-His257
Accession # ABM86208
Pro17-His257
Accession # ABM86208
Specificity
Detects human LILRB4/CD85k/ILT3 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) ILT4 and rhILT5 and 5% cross-reactivity with rhILT2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human LILRB4/CD85k/ILT3 Biotinylated Antibody
Detection of Human ILT3/CD85k/LILRB4 by Flow Cytometry
CSF monocytes express high levels of ILT3.(A) Paired blood and CSF samples were analyzed for expression of ILT3 on CD14+ monocytes by flow cytometry. The specific fluorescent index is shown. Patients were categorized according to their diagnosis as having non-inflammatory neurological diseases (NIND, n = 14; marked by dot) or inflammatory neurological disease (IND, n = 8) including 5 MS patients (marked by x) and 3 patients with other inflammatory neurological disease (diamond). A Mann-Whitney U test was performed to assess statistical significance. (B) Histogram of a representative staining in a patient with NIND. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25551576), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human ILT3/CD85k/LILRB4 by Flow Cytometry
CSF monocytes express high levels of ILT3.(A) Paired blood and CSF samples were analyzed for expression of ILT3 on CD14+ monocytes by flow cytometry. The specific fluorescent index is shown. Patients were categorized according to their diagnosis as having non-inflammatory neurological diseases (NIND, n = 14; marked by dot) or inflammatory neurological disease (IND, n = 8) including 5 MS patients (marked by x) and 3 patients with other inflammatory neurological disease (diamond). A Mann-Whitney U test was performed to assess statistical significance. (B) Histogram of a representative staining in a patient with NIND. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25551576), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human LILRB4/CD85k/ILT3 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human LILRB4/CD85k/ILT3
Sample: Recombinant Human LILRB4/CD85k/ILT3
Reviewed Applications
Read 1 review rated 4 using BAF2425 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LILRB4/CD85k/ILT3
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
Alternate Names
CD85k, HM18, ILT3, LIR5
Gene Symbol
LILRB4
UniProt
Additional LILRB4/CD85k/ILT3 Products
Product Documents for Human LILRB4/CD85k/ILT3 Biotinylated Antibody
Product Specific Notices for Human LILRB4/CD85k/ILT3 Biotinylated Antibody
For research use only
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