Human Lipase I Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5909

R&D Systems, part of Bio-Techne
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AF5909
AF5909-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Lipase I Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Lipase I
Arg14-His154
Accession # Q6XZB0

Specificity

Detects human Lipase I in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Lipase I Antibody

Detection of Human Lipase I antibody by Western Blot.

Detection of Human Lipase I by Western Blot.

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipase I at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Lipase I Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipase I

Lipase I (Lipase member I; also mPA‑PLA1 beta LPDL and PLA1C) is a 55 kDa member of the PLA1 subfamily, pancreatic lipase family, AB hydrolase superfamily of enzymes. It is expressed by sperm, where it is apparently secreted and subsequently binds to membrane-associated HSPGs. Lipase I hydrolyzes phosphatidic acid to generate 2‑acyl‑LPA, which then activates one of four G-protein coupled receptors. Human Lipase I precursor is 460 amino acids (aa) in length. It contains a 15 aa signal sequence, followed by an active catalytic site composed of Ser159, Asp183 and His268. Lipase I is known to be phosphorylated on Ser25. There are two isoform variants, both of which show a 34 aa substitution for aa 1‑13, and only one of which shows an eight aa substitution for aa 245‑460. Over aa 14‑154, human Lipase I shares 61% aa identity with mouse Lipase I.

Alternate Names

CT17, LIPI, LPDL, PRED5

Entrez Gene IDs

149998 (Human); 288322 (Rat)

Gene Symbol

LIPI

UniProt

Q6XZB0 (Human)

Additional Lipase I Products

Product Documents for Human Lipase I Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Lipase I Antibody

For research use only

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