Human Lipocalin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1708
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Lipocalin-1 (R&D Systems, Catalog # 1708-PI)
His19-Asp176
Accession # P31025
His19-Asp176
Accession # P31025
Specificity
Detects human Lipocalin-1 in direct ELISAs and Western blots. In these formats, less than 5% cross‑reactivity with recombinant human Lipocalin-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Lipocalin-1 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Lipocalin-1 (Catalog # 1708-PI)
Sample: Recombinant Human Lipocalin-1 (Catalog # 1708-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipocalin-1
Lipocalin-1, also known as tear prealbumin or von Ebner’s gland protein (VEGP), is encoded by the LCN1 gene (1 - 3). It is a member of the Lipocalin superfamily that binds many different classes of lipophylic chemicals (4). Lipocalin-1 contains three sequence motifs similar to the cystatins, a superfamily of cysteine protease inhibitors (5). In fact, it has been suggested that Lipocalin-1 is a physiological inhibitor of cysteine proteases and plays a role in the control of inflammatory processes in oral and ocular tissues (5). rhLipocalin-1 corresponds to the mature and secreted protein. It is a weak inhibitor of cysteine proteases such as cathepsin V, which is similar to rhCystatin S (R&D Systems, Catalog # 1296-PI).
References
- Redl, B. et al. (1992) J. Biol. Chem. 267:20282.
- Blaker, M. et al. (1993) Biochim. Biophys. Acta 1172:131.
- Lassagne, H. and A.M. Gachon (1993) Exp. Eye Res. 56:605.
- Redl, B. et al. (2000) Biochim. Biophys. Acta 1482:241.
- van’t Hof, W. et al. (1997) J. Biol. Chem. 272:1837.
Alternate Names
LCN1, Lipocalin1, Tear Prealbumin, VEGP
Entrez Gene IDs
3933 (Human)
Gene Symbol
LCN1
UniProt
Additional Lipocalin-1 Products
Product Documents for Human Lipocalin-1 Antibody
Product Specific Notices for Human Lipocalin-1 Antibody
For research use only
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