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Human LMO2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2726

R&D Systems, part of Bio-Techne
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AF2726
AF2726-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human LMO2
Ser2-Ile158
Accession # P25791

Specificity

Detects human LMO2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LMO2 Antibody

Detection of LMO2 antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of LMO2 in K562 Human Cell Line by Flow Cytometry.

K562 chronic myelogenous leukemia human cell line was stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.
Detection of LMO2 antibody in Human PBMC antibody by Flow Cytometry

Detection of LMO2 in Human PBMC by Flow Cytometry

Human PBMC were stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.
LMO2 antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

LMO2 in K562 Human Cell Line.

LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human LMO2 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line and human PBMC

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human LMO2
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMO2

LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Entrez Gene IDs

4005 (Human)

Gene Symbol

LMO2

UniProt

Additional LMO2 Products

Product Documents for Human LMO2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LMO2 Antibody

For research use only

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