Human LMO2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2726
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human LMO2
Ser2-Ile158
Accession # P25791
Ser2-Ile158
Accession # P25791
Specificity
Detects human LMO2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human LMO2 Antibody
Detection of LMO2 in K562 Human Cell Line by Flow Cytometry.
K562 chronic myelogenous leukemia human cell line was stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.
Detection of LMO2 in Human PBMC by Flow Cytometry
Human PBMC were stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.
LMO2 in K562 Human Cell Line.
LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human LMO2 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line and human PBMC
Sample: K562 human chronic myelogenous leukemia cell line and human PBMC
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human LMO2
Sample: Recombinant Human LMO2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LMO2
Long Name
LIM Domain Only 2
Alternate Names
RBTN2, RBTNL1, RHOM2, TTG2
Entrez Gene IDs
4005 (Human)
Gene Symbol
LMO2
UniProt
Additional LMO2 Products
Product Documents for Human LMO2 Antibody
Product Specific Notices for Human LMO2 Antibody
For research use only
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