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Human LRP-4 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5948G

R&D Systems, part of Bio-Techne
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FAB5948G-100UG

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 741704

Product Specifications

Immunogen

E. coli-derived recombinant human LRP-4
Gly18-Ser344
Accession # O75096

Specificity

Detects human LRP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) LRP-5 or rhLRP-6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human LRP-4 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: U-2 OS human osteosarcoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LRP-4

LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells and binds multiple ligands including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905). In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 97% aa identity with both mouse and rat LRP‑4. MAB5948 detects the correct rat band in Western Blot. Immunohistochemistry was not tested in rat samples.  

Long Name

LDL Receptor-related Protein 4

Alternate Names

LRP10, LRP4

Entrez Gene IDs

4038 (Human); 228357 (Mouse); 83469 (Rat)

Gene Symbol

LRP4

UniProt

Additional LRP-4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LRP-4 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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