Detects human LRP-6 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) LRP-6 and no cross-reactivity with recombinant human (rh) LRP-1, -4, -5, rhLRP-6 (intracellular domain), or rhLDL R is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human LRP-6 APC-conjugated Antibody

Detection of LRP‑6 in MDA‑MB‑231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human LRP-6 APC-conjugated Monoclonal Antibody (Catalog # FAB1505A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LRP-6 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: MDA‑MB‑231 human breast cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LRP-6

LRP-6 (Low-density lipoprotein receptor-related protein 6) is a 200-210 kDa member of the LDLR family of proteins. It is widely expressed, and serves as a coreceptor for both Wnt and parathyroid hormone. In the Wnt system, LRP-6 associates with select Fzd multipass receptors; in the PTH system, LRP-6 complexes with PTH1R. Mature human LRP-6 is a 1594 amino acid (aa) type I transmembrane glycoprotein. It contains a 1351 aa extracellular region (aa 20-1370) plus a 220 aa cytoplasmic domain (aa 1394-1613). The cytoplasmic domain contains two palmitoylation sites, one ubiquitination residue, and multiple phosphorylation motifs.

Long Name

LDL Receptor-related Protein 6

Alternate Names

ADCAD2, FLJ90062, FLJ90421, LRP6

Entrez Gene IDs

4040 (Human); 16974 (Mouse)

Gene Symbol

LRP6

UniProt

O75581

Additional LRP-6 Products

Product Documents for Human LRP-6 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human LRP-6 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human LRP-6 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1505A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications