Human LYPD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6855
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human LYPD1
Cys54-Ser116
Accession # Q8N2G4
Cys54-Ser116
Accession # Q8N2G4
Specificity
Detects human LYPD1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human LYPD3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human LYPD1 Antibody
LYPD1 in Human Brain.
LYPD1 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human LYPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6855) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human LYPD1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LYPD1
LYPD1 (Ly6/PLAUR-domain containing protein 1; also Lynx2 and PHTS) is a 15-20 kDa member of the Ly6 family of GPI-anchored proteins. It is expressed on postmitotic neurons in multiple locations, performing multiple functions. Two suggested functions include the ability to 1) block cell proliferation, and 2) bind to acetylcholine receptors, promoting their desensitization. Human LYPD1 preproprecursor is 141 amino acids (aa) in length. It contains a signal sequence (aa 1-20), a 97 aa mature region (aa 21-117), and a cleavable prodomain that, when cleaved, generates a GPI-linked form (aa 118-141). The mature molecule contains one Ly6 domain (aa 25-107). There are multiple potential isoform variants. One possesses an alternative start site at Met53, a second contains a 21 aa substitution for aa 1-5, a third shows a 21 aa substitution for aa 64-141, and a fourth possesses a 29 aa substitution for aa 65-141. Over aa 54-116, human and mouse LYPD1 are identical in aa sequence.
Long Name
Ly6/PLAUR Domain-containing Protein 1
Alternate Names
LYPDC1, PHTS
Gene Symbol
LYPD1
UniProt
Additional LYPD1 Products
Product Documents for Human LYPD1 Antibody
Product Specific Notices for Human LYPD1 Antibody
For research use only
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