Human Lysine Hydroxylase 2/PLOD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4445

R&D Systems, part of Bio-Techne
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MAB4445
MAB4445-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 408105

View all Lysine Hydroxylase 2/PLOD2 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lysine Hydroxylase 2/PLOD2
Ala26-Pro758
Accession # NP_891988

Specificity

Detects human Lysine Hydroxylase 2/PLOD2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Lysine Hydroxylase 2/PLOD2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Lysine Hydroxylase 2, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Lysine Hydroxylase 2
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB4445 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysine Hydroxylase 2/PLOD2

Lysine Hydroxylase 2 (LH2) also known as PLOD2 (procollagen-lysine, 2 oxoglutarate 5 dioxygenase 2) is a membrane-bound homodimeric enzyme localized to the cisternae of the rough ER. The enzyme catalyzes the hydroxylation of lysine residues in collagen-like peptides, which in turn serves as sites for carbohydrate attachment in collagen. Two alternatively spliced forms have 737 or 758 amino acids (aa). Human LH2 (758 aa form) shares 92.5% aa sequence identity with the appropriate isoform of mouse and rat LH2.

Alternate Names

LH2, PLOD2, TLH

Entrez Gene IDs

5352 (Human); 26432 (Mouse); 300901 (Rat)

Gene Symbol

PLOD2

UniProt

NP_891988

Additional Lysine Hydroxylase 2/PLOD2 Products

Product Documents for Human Lysine Hydroxylase 2/PLOD2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Lysine Hydroxylase 2/PLOD2 Antibody

For research use only

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