Human MAD1L1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4194
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MAD1L1
Met1-Asp350
Accession # Q9Y6D9
Met1-Asp350
Accession # Q9Y6D9
Specificity
Detects human MAD1L1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MAD1L1 Antibody
Detection of Human MAD1L1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human MAD1L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4194) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MAD1L1 at approximately 90 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human MAD1L1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAD1L1
MAD1L1 (mitotic arrest deficient 1-like 1) is a member of the spindle-assembly checkpoint (SAC) which prevents anaphase from occurring until all chromosomes are properly aligned at the metaphase plate. Upon checkpoint activation, MAD1L1 recruits MAD2 to unattached kinetochores, promoting the binding of MAD2 to CDC20, the activator for the anaphase-promoting complex. MAD1L1 has been implicated to have tumor-suppressing functions, but in small-cell lung cancer cell lines, the MAD1L1 gene is the most frequent copy number gain, suggesting a more complex role.
Long Name
MAD1 Mitotic Arrest Deficient-Like 1
Alternate Names
HsMAD1, MAD1, PIG9, TP53I9, TXBP181
Gene Symbol
MAD1L1
UniProt
Additional MAD1L1 Products
Product Documents for Human MAD1L1 Antibody
Product Specific Notices for Human MAD1L1 Antibody
For research use only
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