Human MafG Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3924
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 370412
Product Specifications
Immunogen
E. coli-derived recombinant human MafG
Met1-Ser162
Accession # O15525
Met1-Ser162
Accession # O15525
Specificity
Detects human MafG in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafF or rhMafK is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human MafG Antibody
Detection of Human MafG by Western Blot.
Western blot shows recombinant human MafF, MafG, and MafK (5 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human MafG by Western Blot.
Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human MafG Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HL-60 human acute promyelocytic leukemia cell line and Ramos human Burkitt's lymphoma cell line
Sample: HL-60 human acute promyelocytic leukemia cell line and Ramos human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MafG
Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members.
Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog G
Alternate Names
basic leucine zipper transcription factor MafG, hMAF, MGC13090, MGC20149, transcription factor MafG, v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)
Gene Symbol
MAFG
UniProt
Additional MafG Products
Product Documents for Human MafG Antibody
Product Specific Notices for Human MafG Antibody
For research use only
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