Human MAGP-2/MFAP5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4914
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MAGP-2/MFAP5
Ile22-Leu173
Accession # Q13361
Ile22-Leu173
Accession # Q13361
Specificity
Detects human MAGP-2/MFAP5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human MAGP-1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human MAGP-2/MFAP5 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human MAGP-2/MFAP5
Sample: Recombinant Human MAGP-2/MFAP5
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAGP-2/MFAP5
MAGP-2 (Microfibril-associated glycoprotein 2; also MFAP-5) is a 25 kDa member of the MFAP family of proteins. It is secreted by smooth muscle cells and has multiple functions. Through its binding to EGF repeats, MAGP-2 mediates the release of Jagged1 and Notch extracellular domains, and also binds to matrix microfibrillar proteins, inducing elastin fiber formation. Mature human MAGP-2 is 152 amino acids (aa) in length. It contains an RDG motif (aa 30‑32) and a central Cys-rich domain (aa 84‑140) that participates in covalent binding to fibrillin microfibrils. One potential splice variant exists that shows a 10 aa deletion between aa 73‑82. Mature human MAGP-2 is 86% and 87% aa identical to mouse and canine MAGP-2, respectively.
Long Name
Microfibril-associated Glycoprotein 2
Alternate Names
MAGP2, MFAP5, MP25
Gene Symbol
MFAP5
UniProt
Additional MAGP-2/MFAP5 Products
Product Documents for Human MAGP-2/MFAP5 Antibody
Product Specific Notices for Human MAGP-2/MFAP5 Antibody
For research use only
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