Human MASTL Antibody

R&D Systems, part of Bio-Techne | Catalog # AF10022

R&D Systems, part of Bio-Techne
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AF10022-100
AF10022-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all MASTL Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MASTL.
Lys39-Lys158
Accession # Q96GX5

Specificity

Detects human MASTL in direct ELISA.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MASTL Antibody

MASTL antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

MASTL in HeLa Human Cell Line.

MASTL was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human MASTL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10022) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human MASTL Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MASTL

MAST-L is a ubiquitous microtubule associated serine/threonine kinase which plays a key role in mitosis. During entry into mitosis, CDK1 and MASTL kinase repress the activity of mitotic protein phosphatases PP1 and PP2A. Mitotic exit requires partial dephosphorylation of MASTL by PP1, followed by dephosphorylation of PP2A. PP1 is partially reactivated by decreases in CDK1 activity. PP1 then reactivates PP1A. Mutations of MASTL have been associated with autosomal dominant thrombocytopenia, a decrease in the number of platelets circulating in blood. MASTL kinase is frequently upregulated in several cancers, and is correlated with cancer progression, poor patient survival and tumor recurrence. Ectopic expression of MASTL in tumor cells has been shown to promote cell proliferation, and MASTL has been identified as a target for therapeutic intervention.

Long Name

MASTL

Alternate Names

Greatwall, THC2

Entrez Gene IDs

84930 (Human); 67121 (Mouse)

Gene Symbol

MASTL

UniProt

Q96GX5

Additional MASTL Products

Product Documents for Human MASTL Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MASTL Antibody

For research use only

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