Human Megalin/LRP2 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9578P

R&D Systems, part of Bio-Techne
Discontinued Product
FAB9578P has been discontinued. View all Megalin/LRP2 products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 545606

View all Megalin/LRP2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Megalin/LRP2
Pro3510-Lys3964
Accession # P98164

Specificity

Detects human Megalin/LRP2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Megalin/LRP2 PE-conjugated Antibody

Detection of Megalin/LRP2 in NSO cells transfected with Human LRP2 cells by Flow Cytometry.

NSO cells transfected with Human LRP2 were stained with Mouse Anti-Human Megalin/LRP2 PE-conjugated Monoclonal Antibody (Catalog # FAB9578P, filled histogram) or irrelevant transfectant. To facilitate intracellular staining, cells were fixed with FC004 and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.

Applications for Human Megalin/LRP2 PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: NSO cells transfected with Human LRP2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Megalin/LRP2

Megalin, also known as the low-density lipoprotein receptor-related protein 2 (LRP2), is a large type I transmembrane cell surface protein. This glycoprotein is a multi‑ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney (1). The Megalin protein is critical for the re-uptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling. Mutations in this gene cause Donnai-Barrow Syndrome (DBS) and Facio-Oculoacoustico-Renal Syndrome (FOAR) (1). Megalin is consisting of a 25 amino acid (aa) probable N‑terminal signal peptide sequence, a 4400 aa extracellular region, a 22 aa single transmembrane domain, and a 213 aa C‑terminal cytoplasmic tail. The entire extracellular region is made up of 36 class A motifs of putative ligand-binding domains arranged in four distinct clusters, 16 growth factor repeats separated by 8 YWTD spacer regions, and 1 epidermal growth factor-like repeat (2). The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase (3). The amino acid 3510-3964 encodes the fourth class A motif cluster in human Megalin, termed Megalin C4. Human Megalin C4 shares 77% and 74% identity with mouse and rat Megalin C4.

References

  1. Christensen, E. I. and Birn, H. (2002) Nat. Rev. Mol. Cell Biol 3:256.
  2. Saito, A. et al. (1994) Proc.Natl. Aca. Sci. U. S. A. 91:9725.
  3. Kantarci, S. et al. (2007) Nat. Genet 39:957.

Long Name

LDL Receptor-related Protein 2

Alternate Names

DBS, gp330, LRP-2, LRP2

Entrez Gene IDs

4036 (Human); 14725 (Mouse); 29216 (Rat)

Gene Symbol

LRP2

UniProt

P98164

Additional Megalin/LRP2 Products

Product Documents for Human Megalin/LRP2 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Megalin/LRP2 PE-conjugated Antibody

For research use only

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