HEK293 human embryonic kidney cell line transfected with human Melanocortin-5 R/MC5R and eGFP was stained with and either (A) Mouse Anti-Human Melanocortin-5 R/MC5R Monoclonal Antibody (Catalog # MAB8205) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human Melanocortin-5 R/MC5R Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human Melanocortin-5 R/MC5R and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melanocortin-5 R/MC5R

Melanocortin 5R (MC5R) is a member of the melanocortin receptor family. The 325 amino acid G-protein-coupled 7-transmembrane glycoprotein is expressed in skeletal muscle, where engagement by a-melanocyte stimulating hormone increases glucose uptake and stimulates a thermogenic response. Expression is also found in sebaceous glands and other skin structures, and on selected hematopoietic cell lines. Human MC5R shares 80-81% amino acid sequence identity with mouse and rat MC5R.

Long Name

Melanocortin 5 Receptor

Alternate Names

MC2, MC5, MC5R, Melanocortin-5R, Melanocortin5R

Entrez Gene IDs

4161 (Human); 17203 (Mouse); 25726 (Rat)

Gene Symbol

MC5R

UniProt

P33032 (Human)

Additional Melanocortin-5 R/MC5R Products

Product Documents for Human Melanocortin-5 R/MC5R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Melanocortin-5 R/MC5R Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Melanocortin-5 R/MC5R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8205

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Melanocortin-5 R/MC5R Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Melanocortin-5 R/MC5R Antibody

Detection of Melanocortin-5 R/MC5R in HEK293 Human Cell Line Transfected with Human Melanocortin-5 R/MC5R and eGFP by Flow Cytometry.