Human Melanopsin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10497
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 628737
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Melanopsin
Accession # Q9UHM6
Accession # Q9UHM6
Specificity
Detects human Melanopsin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Melanopsin Antibody
Detection of Human Melanopsin by Western Blot.
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human Melanopsin. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Melanopsin Monoclonal Antibody (Catalog # MAB10497) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Melanopsin at approximately 200-350 kDa (as indicated). GAPDH is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Melanopsin in HEK293 Human Cell Line Transfected with Human Melanopsin and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) Human Melanopsin or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Melanopsin Monoclonal Antibody (Catalog # MAB10497) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Human Melanopsin Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Melanopsin and eGFP
Sample: HEK293 Human Cell Line Transfected with Human Melanopsin and eGFP
Western Blot
2 µg/mL
Sample: NS0 mouse myeloma cell line transfected with human Melanopsin
Sample: NS0 mouse myeloma cell line transfected with human Melanopsin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Melanopsin
Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.
Long Name
Melanopsin
Alternate Names
OPN4, Opsin-4
Gene Symbol
OPN4
UniProt
Additional Melanopsin Products
Product Documents for Human Melanopsin Antibody
Product Specific Notices for Human Melanopsin Antibody
For research use only
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