Human Meprin alpha Subunit/MEP1A Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC3220S
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Val22-Gln601
Accession # AAA21338
Specificity
Clonality
Host
Isotype
Applications for Human Meprin alpha Subunit/MEP1A Alexa Fluor® 750-conjugated Antibody
Intracellular Staining by Flow Cytometry
Sample: HEK293 human embryonic kidney cell line fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: Meprin alpha Subunit/MEP1A
Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid (aa) sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). Human Meprin alpha subunit consists of a signal peptide (aa 1 to 21), a pro region (aa 22 to 65), and a mature chain (aa 66 to 746) containing the following domains: catalytic (aa 62 to 263), MAM (aa 264 to 433), MATH (aa 434 to 593), EGF-like (aa 670 to 710), transmembrane (aa 713 to 740), and cytoplasmic (aa 741 to 746).
References
- Bond, J.S. and Beynon, R.J. (1995) Protein Sci. 4:1247.
- Stocker, W. et al. (1995) Protein Sci. 4:823.
- Bertenshaw, G.P., et al. (2001) J. Biol. Chem. 276:13248.
- Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.
Alternate Names
Gene Symbol
UniProt
Additional Meprin alpha Subunit/MEP1A Products
Product Specific Notices for Human Meprin alpha Subunit/MEP1A Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only