Human Methionine Aminopeptidase 1/METAP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3537
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 405419
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Methionine Aminopeptidase 1/METAP1
His52-Phe386
Accession # P53582
His52-Phe386
Accession # P53582
Specificity
Detects human Methionine Aminopeptidase 1/METAP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Methionine Aminopeptidase 2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Methionine Aminopeptidase 1/METAP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN)
Sample: Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Methionine Aminopeptidase 1/METAP1
The human METAP1 gene encodes Methionine Aminopeptidase 1, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). Inhibition of METAPs provides a novel strategy in developing anti-cancer drugs (3). The amino acid sequence of human METAP1 is 99%, 98%, 95% and 64% identical to that of chimpanzee, rat/mouse, chicken and fruit fly.
References
- Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
- Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
- Liu, S. et al. (1998) Science 282:1324.
Alternate Names
MAP1, MetAP1
Gene Symbol
METAP1
UniProt
Additional Methionine Aminopeptidase 1/METAP1 Products
Product Documents for Human Methionine Aminopeptidase 1/METAP1 Antibody
Product Specific Notices for Human Methionine Aminopeptidase 1/METAP1 Antibody
For research use only
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