Human Methionine Aminopeptidase 1/
METAP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3537
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Methionine Aminopeptidase 1/METAP1
His52-Phe386
Accession # P53582
His52-Phe386
Accession # P53582
Specificity
Detects human Methionine Aminopeptidase 1/METAP1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Methionine Aminopeptidase 1/METAP1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN)
Sample: Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Methionine Aminopeptidase 1/METAP1
The human METAP1 gene encodes Methionine Aminopeptidase 1, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). Inhibition of METAPs provides a novel strategy in developing anti-cancer drugs (3). The purified rhMETAP1 consists of amino acid residues 52/53 to 386. A previous report showed that the N-terminal 89 amino acid region was not essential for catalytic activity (4).
References
- Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
- Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
- Liu, S. et al. (1998) Science 282:1324.
- Addlagatta, A. et al. (2005) Biochemistry 44:14741.
Alternate Names
MAP1, MetAP1
Gene Symbol
METAP1
UniProt
Additional Methionine Aminopeptidase 1/METAP1 Products
Product Documents for Human Methionine Aminopeptidase 1/METAP1 Antibody
Product Specific Notices for Human Methionine Aminopeptidase 1/METAP1 Antibody
For research use only
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