Human Methionine Aminopeptidase 1D/MAP1D Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4714
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Methionine Aminopeptidase 1D/MAP1D
Arg44-Ala335
Accession # Q6UB28
Arg44-Ala335
Accession # Q6UB28
Specificity
Detects human Methionine Aminopeptidase 1D/MAP1D in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Methionine Aminopeptidase 1D/MAP1D Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 1D/MAP1D (Catalog # 4714-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 1D/MAP1D (Catalog # 4714-ZN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Methionine Aminopeptidase 1D/MAP1D (Catalog # 4714-ZN)
Sample: Recombinant Human Methionine Aminopeptidase 1D/MAP1D (Catalog # 4714-ZN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Methionine Aminopeptidase 1D/MAP1D
Methionine Aminopeptidase 1D (MAP1D) is a member of the Methionine Aminopeptidase family (MetAPs) (1). MetAPs catalyze the removal of the initiator methionine residue from nascent peptides (2) and are essential for cell growth (3). MAP1D is localized in mitochondria (4) and is over-expressed in colon cancer cell lines and colon tumors (1). The N-terminal leader peptide (residue 1‑43) of MAP1D has been shown to be responsible for targeting to mitochondria (4).
References
- Leszczyniecka, M. et al. (2006) Oncogene 25:3471.
- Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
- Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
- Serero, A. et al. (2003) J. Biol. Chem. 278:52953.
Alternate Names
MAP1D, MetAP1D
Entrez Gene IDs
254042 (Human)
Gene Symbol
METAP1D
UniProt
Additional Methionine Aminopeptidase 1D/MAP1D Products
Product Documents for Human Methionine Aminopeptidase 1D/MAP1D Antibody
Product Specific Notices for Human Methionine Aminopeptidase 1D/MAP1D Antibody
For research use only
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