Human mGluR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4676
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 455310
Product Summary for Human mGluR2 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human mGluR2
Glu19-Ser498
Accession # Q14416
Glu19-Ser498
Accession # Q14416
Specificity
Detects human mGluR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with the N-terminal regions of recombinant human mGluR1, -3, -4, -5, -7, or -8 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human mGluR2 Antibody
Detection of mGluR2 in U‑118‑MG Human Cell Line by Flow Cytometry.
U-118-MG human glioblastoma/ astrocytoma cell line was stained with Mouse Anti-Human mGluR2 Monoclonal Antibody (Catalog # MAB4676, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permea-bilized with saponin.Applications for Human mGluR2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: U-118-MG human glioblastoma/astrocytoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: U-118-MG human glioblastoma/astrocytoma cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human mGluR2
Sample: Recombinant Human mGluR2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: mGluR2
Metabotropic glutamate receptor 2 (mGluR2) is an 872 aa, predicted 96 kDa multipass G protein coupled inhibitory receptor belonging to group II of the metabotropic glutamate receptor family. It is localized largely on the presynaptic side of glutamatergic and other neurotransmitter synapses in areas of the forebrain. mGluR2 activity is potentially involved in some anxiety disorders. The long N‑terminal extracellular region of human mGluR2 (aa 1‑498) shares 97% aa identity with either mouse or rat mGluR2.
Long Name
Metabotropic Glutamate Receptor 2
Alternate Names
GPRC1B, GRM2, mGluR7
Gene Symbol
GRM2
UniProt
Additional mGluR2 Products
Product Documents for Human mGluR2 Antibody
Product Specific Notices for Human mGluR2 Antibody
For research use only
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