Human mGluR3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB46681
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 530510
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human mGluR3
Asp25-Ser507
Accession # Q14832
Asp25-Ser507
Accession # Q14832
Specificity
Detects human mGluR3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human mGluR3 Antibody
Detection of mGluR3 in HEK293 Human Cell Line Transfected with Human mGluR3 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human mGluR3 and eGFP was stained with (A) Mouse Anti-Human mGluR3 Monoclonal Antibody (Catalog # MAB46681) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Human mGluR3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human MGLUR3 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human MGLUR3 and eGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: mGluR3
Metabotropic glutamate receptor 3 (mGluR3) is a 90-100 kDa, 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. It is a presynaptic receptor expressed on both neurons and glia, whose activation reduces adenylate cyclase activity. Mature human mGluR3 is 857 amino acids in length and contains a 554 amino acid (aa) N-terminal extracellular domain (ECD) (aa 23-576). The ECD binds glutamate and forms homodimers. There is one alternative splice form that is soluble, 515 aa in length and shows a 96 aa substitution for aa 442-879. Over aa 25-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3 and 67% aa sequence identity with hGluR2.
Long Name
Metabotropic Glutamate Receptor 3
Alternate Names
GPRC1C, GRM3
Gene Symbol
GRM3
UniProt
Additional mGluR3 Products
Product Documents for Human mGluR3 Antibody
Product Specific Notices for Human mGluR3 Antibody
For research use only
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