Human Mineralocorticoid R/NR3C2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4369
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 385707
Product Specifications
Immunogen
E. coli-derived recombinant human Mineralocorticoid R/NR3C2
Met1-Leu670
Accession # AAI11759
Met1-Leu670
Accession # AAI11759
Specificity
Detects human Mineralocorticoid R/NR3C2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Mineralocorticoid R/NR3C2 Antibody
Detection of Human Mineralocorticoid R/NR3C2 by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Mineralocorticoid R/NR3C2 Monoclonal Antibody (Catalog # MAB4369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Mineralocorticoid R/NR3C2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human Mineralocorticoid R/NR3C2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
1 µg/mL
Sample: LNCaP human prostate cancer cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line
Sample: LNCaP human prostate cancer cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line
Reviewed Applications
Read 1 review rated 5 using MAB4369 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mineralocorticoid R/NR3C2
Mineralocorticoid Receptor (MR) is a member of the nuclear hormone receptor family. Upon binding of ligand (aldosterone or corticosterone) MR translocates to the nucleus and can form either homo or heterodimers. MR dimers bind to the mineralocorticoid response element (AGAACANNNTGTTCT) to regulate gene transcription. There are four known isoforms of MR.
Long Name
Mineralocorticoid Receptor
Alternate Names
MineralocorticoidR, MLR, NR3C2
Entrez Gene IDs
4306 (Human)
Gene Symbol
NR3C2
UniProt
Additional Mineralocorticoid R/NR3C2 Products
Product Documents for Human Mineralocorticoid R/NR3C2 Antibody
Product Specific Notices for Human Mineralocorticoid R/NR3C2 Antibody
For research use only
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