Human MIS/AMH Propeptide Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2748
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MIS/AMH Propeptide
Leu19-Gln450
Accession # P03971
Leu19-Gln450
Accession # P03971
Specificity
Detects human MIS/AMH Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human MIS is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human MIS/AMH Propeptide Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovary subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human ovary subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
0.1 µg/mL
Sample: Recombinant Human MIS/AMH (Catalog # 1737-MS)
Sample: Recombinant Human MIS/AMH (Catalog # 1737-MS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIS/AMH
MIS, also known as anti-Mullerian Hormone (AMH), belongs to the TGF-beta superfamily. It is synthesized as a 140 kDa homodimeric glycoprotein that undergoes proteolytic cleavage into the bioactive homodimeric 26 kDa carboxy-terminus and the 110 kDa dimeric amino-terminus. After cleavage, the amino-terminal propeptide remains non-covalently associated with the bioactive carboxy-terminal active protein. The amino acid sequence of human and mouse MIS propeptides are 67% identical.
Long Name
Mullerian-inhibiting Substance/Anti-Mullerian Hormone
Alternate Names
AMH
Gene Symbol
AMH
UniProt
Additional MIS/AMH Products
Product Documents for Human MIS/AMH Propeptide Antibody
Product Specific Notices for Human MIS/AMH Propeptide Antibody
For research use only
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