Human Miz-1/ZBTB17 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3760

R&D Systems, part of Bio-Techne
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AF3760
AF3760-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Miz-1/ZBTB17 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Miz-1
Met1-Ala188
Accession # Q13105

Specificity

Detects endogenous human Miz-1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Miz-1/ZBTB17 Antibody

Detection of Human Miz-1/ZBTB17 antibody by Western Blot.

Detection of Human Miz-1/ZBTB17 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human Miz-1/ZBTB17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3760) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Miz-1/ZBTB17 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human Miz-1/ZBTB17 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and CEM human T-lymphoblastoid cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Miz-1/ZBTB17

Miz-1 (Myc-interacting zinc finger protein 1; also ZBTB17) is an 88 kDa, DNA-binding protein that belongs to the Kruppel C2H2-type zinc finger protein family. Human Miz-1 is 803 amino acids (aa) in length, and contains a POZ domain (aa 1 - 104), two Myc-interaction segments (aa 269 - 308 & 637 - 718), and 12 C2H2-type zinc finger motifs (aa 306 - 637); also, there is one alternate splice form that shows an eight aa substitution for Thr769. Miz-1 induces cell arrest at G1, an effect mediated by its activation of the gene coding for p15INK4b. This effect is blocked by Myc, which displaces transcriptional coactivators bound to Miz-1. Notably, although the downregulation of Miz-1 may contribute to Myc-induced cell transformation, the deactivation of Miz-1 is absolutely essential for Myc-induced apoptosis. Over aa 1‑188, human Miz-1 is 85% aa identical to mouse Miz-1.

Long Name

Myc-interacting Zinc Finger Protein-1

Alternate Names

mZ13, ZBTB17, Zfp100, ZNF151

Entrez Gene IDs

7709 (Human); 22642 (Mouse); 313666 (Rat)

Gene Symbol

ZBTB17

UniProt

Q13105

Additional Miz-1/ZBTB17 Products

Product Documents for Human Miz-1/ZBTB17 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Miz-1/ZBTB17 Antibody

For research use only

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