Human MMP-15/MT2-MMP Catalytic Domain Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB916
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 130516
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-15
aa 132-625
aa 132-625
Specificity
Detects human MMP‑15/MT2‑MMP Catalytic Domain in Western blots. In Western blots, no cross-reactivity with the catalytic domain of recombinant human MMP-14 (MT1-MMP) or rhMMP-16 (MT3-MMP) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human MMP-15/MT2-MMP Catalytic Domain Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-15, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-15, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human MMP-15 Catalytic Domain
Sample: Recombinant Human MMP-15 Catalytic Domain
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-15/MT2-MMP
Long Name
Matrix Metalloproteinase 15/Membrane Type 2 MMP
Alternate Names
MMP15, MT2-MMP
Entrez Gene IDs
4324 (Human)
Gene Symbol
MMP15
Additional MMP-15/MT2-MMP Products
Product Documents for Human MMP-15/MT2-MMP Catalytic Domain Antibody
Product Specific Notices for Human MMP-15/MT2-MMP Catalytic Domain Antibody
For research use only
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