PC-3 human prostate cancer cell line was stained with Mouse Anti-Human MMP-16/MT3-MMP Monoclonal Antibody (Catalog # MAB1785, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human MMP-16/MT3-MMP Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: PC‑3 human prostate cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-16/MT3-MMP

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix (ECM). MMP-16 (MT3-MMP) is found in brain, lung, placenta, smooth muscle cells, and malignant tumor tissues including oral melanoma and renal carcinoma (1). MMP-16 has been shown to activate proMMP-2 and degrade various ECM components including native collagens (2, 3). MMP-16 has been proposed to possess the potential to directly enhance the growth and invasiveness of cells in vivo, two critical processes for development and carcinogenesis (4). Structurally, MMP-16 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (1). The structure of the catalytic domain in complex with a hydroxamate inhibitor has been solved (5).

References

Takino, T. et al. (1995) J. Biol. Chem. 270:23013.
Shofuda, K. et al. (1997) J. Biol. Chem. 272:9749.
Shimada, T. et al. (1999) Eur. J. Biochem. 262:907.
Kang, T. et al. (2000) FASEB J. 14:2559.
Lang, R. et al. (2004) J. Mol. Biol. 336:213.

Long Name

Matrix Metalloproteinase 16/Membrane Type 3 MMP

Alternate Names

MMP16, MT3-MMP

Entrez Gene IDs

4325 (Human)

Gene Symbol

MMP16

UniProt

P51512

Additional MMP-16/MT3-MMP Products

Product Documents for Human MMP-16/MT3-MMP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MMP-16/MT3-MMP Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human MMP-16/MT3-MMP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1785

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MMP-16/MT3-MMP Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MMP-16/MT3-MMP Antibody

Detection of MMP-16 in PC-3 Human Cell Line by Flow Cytometry.