Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MMP-25/MT6-MMP Monoclonal Antibody (Catalog # MAB11422, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human MMP-25/MT6-MMP Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-25/MT6-MMP

MMP-25, also known as MT6-MMP or Leukolysin, is a GPI-linked protease expressed by neutrophils. MMP-25 cleaves collagen type 4, gelatin, fibronectin, and fibrin.

Long Name

Matrix Metalloproteinase 25/Membrane Type 6 MMP

Alternate Names

Leukolysin, MMP25, MT6-MMP

Entrez Gene IDs

64386 (Human)

Gene Symbol

MMP25

UniProt

Q9NPA2

Additional MMP-25/MT6-MMP Products

Product Documents for Human MMP-25/MT6-MMP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MMP-25/MT6-MMP Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human MMP-25/MT6-MMP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11422

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MMP-25/MT6-MMP Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MMP-25/MT6-MMP Antibody

Detection of MMP‑25/MT6‑MMP in Jurkat Human Cell Line by Flow Cytometry.