Human MMP‑25/MT6-MMP Hemopexin Domain Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11421
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 141825
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-25
Pro314-Cys508
Accession # Q9NPA2
Pro314-Cys508
Accession # Q9NPA2
Specificity
Detects human MMP‑25/MT6-MMP Hemopexin Domain in Western blots. It recognizes E. coli-expressed hemopexin domain andNS0‑expressed recombinant human MMP-25.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MMP‑25/MT6-MMP Hemopexin Domain Antibody
MMP‑25/MT6‑MMP in Human Lung.
MMP-25/MT6-MMP was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human MMP-25/MT6-MMP Hemopexin Domain Monoclonal Antibody (Catalog # MAB11421) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MMP‑25/MT6-MMP Hemopexin Domain Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung
Sample: Immersion fixed paraffin-embedded sections of human lung
Western Blot
1 µg/mL
Sample: Human MMP-25/MT6-MMP Hemopexin Domain
Sample: Human MMP-25/MT6-MMP Hemopexin Domain
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB11421 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-25/MT6-MMP
Matrix Metalloproteinase 25 (MMP-25), also known as MT6-MMP or Leukolysin, is a GPI-linked protease expressed by neutrophils. The extracellular domain of MMP-25 consists of a pro region, a catalytic domain and a hemopexin domain. MMP-25 cleaves collagen type 4, gelatin, fibronectin and fibrin.
Long Name
Matrix Metalloproteinase 25/Membrane Type 6 MMP
Alternate Names
Leukolysin, MMP25, MT6-MMP
Entrez Gene IDs
64386 (Human)
Gene Symbol
MMP25
UniProt
Additional MMP-25/MT6-MMP Products
Product Documents for Human MMP‑25/MT6-MMP Hemopexin Domain Antibody
Product Specific Notices for Human MMP‑25/MT6-MMP Hemopexin Domain Antibody
For research use only
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