Human/Mouse/Bovine Integrin alpha6/CD49f Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB13501
Clone GoH3 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine
Cited:
Human, Mouse, Porcine
Applications
Validated:
Blockade of Receptor-ligand Interaction, CyTOF-reported, Flow Cytometry, Immunohistochemistry
Cited:
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # GoH3
Product Specifications
Immunogen
Mouse mammary tumor cells
Specificity
Detects human, mouse, and bovine Integrin alpha6/CD49f. Recognizes an epitope in the extracellular domain of the Integrin alpha6 subunit.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human/Mouse/Bovine Integrin alpha6/CD49f Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Sonnenberg, A. et al. (1986) J. Histochem. Cytochem. 34:1037. Knapp, W.B. et al. Eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York. Aumailley, M.R. et al. (1990) Exp. Cell Res. 188:55.
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells
Sample: Human peripheral blood mononuclear cells
Immunohistochemistry
Sonnenberg, A. et al. (1986) J. Histochem. Cytochem. 34:1037.
Reviewed Applications
Read 4 reviews rated 5 using MAB13501 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 6/CD49f
The Integrin alpha6 subunit, also known as CD49f and VLA-6 alpha subunit, forms a heterodimer with Integrin beta1 (CD29) or beta4 (CD104) subunits and recognizes laminin as a ligand.
Alternate Names
CD49f, ITGA6, Platelet gpl, VLA-6
Gene Symbol
ITGA6
Additional Integrin alpha 6/CD49f Products
Product Documents for Human/Mouse/Bovine Integrin alpha6/CD49f Antibody
Product Specific Notices for Human/Mouse/Bovine Integrin alpha6/CD49f Antibody
For research use only
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