Human/Mouse CDC73 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5508

R&D Systems, part of Bio-Techne
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AF5508
AF5508-SP
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CDC73 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CDC73
Met1-Glu260
Accession # Q6P1J9

Specificity

Detects human and mouse CDC73 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse CDC73 Antibody

Detection of Human and Mouse CDC73 antibody by Western Blot.

Detection of Human and Mouse CDC73 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDC73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for CDC73 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse CDC73 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDC73

CDC73 (also Parafibromin, HRPT2, and C1orf28) is a 60‑65 kDa member of the Cell Division Cycle family of proteins. It is found in adrenal, parathyroid and kidney, and serves as a regulator of Wnt signaling and a tumor suppressor by blocking c-myc expression. Human CDC73 is 531 amino acids (aa) in length. It contains multiple NLSs (aa 125‑139, 76‑92, and 393‑410) and a beta-catenin interaction sequence (aa 218‑263). There are at least two potential splice variants. One shows a five Lys substitution for aa 300‑531, while a second shows a 19 aa substitution for aa 298‑531. Full-length human and mouse CDC73 are completely (100%) identical in primary aa sequence.

Long Name

Cell Division Cycle 73

Alternate Names

HPT-JT, HRPT2, Parafibromin

Entrez Gene IDs

79577 (Human); 214498 (Mouse); 304832 (Rat)

Gene Symbol

CDC73

UniProt

Q6P1J9

Additional CDC73 Products

Product Documents for Human/Mouse CDC73 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse CDC73 Antibody

For research use only

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