Human/Mouse COX-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB37401
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 596002
Product Specifications
Immunogen
E.coli-derived recombinant human COX-1
Asn381-Leu599
Accession # P23219
Asn381-Leu599
Accession # P23219
Specificity
Detects human COX-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) COX-4I1 and no cross-reactivity with rhCOX-1 or rhCOX-4I2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse COX-1 Antibody
Detection of Human and Mouse COX‑1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and B16-F10 mouse melanoma cell line. PVDF Membrane was probed with 2 µg/mL of Human COX-1 Monoclonal Antibody (Catalog # MAB37401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for COX-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse COX-1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and B16‑F10 mouse melanoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and B16‑F10 mouse melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: COX-1
COX-1 is an approximately 70 kDa protein that is associated with microsomal membranes. COX-1 participates in the synthesis of prostaglandins and is the enzymatic target of non-steroidal anti-inflammatory drugs. A splice variant of COX-1 is generated by internal deletion of 37 residues. Human COX-1 shares 88%‑90% amino acid sequence identity with mouse and rat COX‑1.
Long Name
Cyclooxygenase 1
Alternate Names
COX1, PTGS1
Gene Symbol
PTGS1
UniProt
Additional COX-1 Products
Product Documents for Human/Mouse COX-1 Antibody
Product Specific Notices for Human/Mouse COX-1 Antibody
For research use only
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