Human/Mouse Crk Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4316
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 463414
Product Summary for Human/Mouse Crk Antibody
Immunogen
E. coli-derived recombinant human Crk
Gln205-Ser304
Accession # P46108
Gln205-Ser304
Accession # P46108
Specificity
Detects human Crk in direct ELISAs and human and mouse Crk in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse Crk Antibody
Detection of Human and Mouse Crk by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Crk Monoclonal Antibody (Catalog # MAB4316) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Crk at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse Crk Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and MOLT‑4 human acute lymphoblastic leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and MOLT‑4 human acute lymphoblastic leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Crk
Crk belongs to a family of adaptor molecules that are widely expressed in numerous tissues. It was originally isolated from a chicken tumor sample, and despite lacking enzymatic activity, this protein is involved in many signal transduction pathways leading to phosphorylation of multiple proteins. Crk forms multi protein complexes via it’s SH2 (aa 13-118) and SH3 (aa 132-192 and aa 237-296) domains leading to activation of downstream molecules involved in growth, differentiation, cell adhesion and migration. Alternate splicing generates an additional isoform that lacks aa 205-304.
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog
Alternate Names
adapter molecule crk, avian sarcoma virus CT10 (v-crk) oncogene homolog, CRKII, p38, Proto-oncogene c-Crk, v-crk avian sarcoma virus CT10 oncogene homolog, v-crk sarcoma virus CT10 oncogene homolog (avian)
Gene Symbol
CRK
UniProt
Additional Crk Products
Product Documents for Human/Mouse Crk Antibody
Product Specific Notices for Human/Mouse Crk Antibody
For research use only
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