Human/Mouse DACT3/Dapper 3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5767
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse DACT3/Dapper 3
Pro486-Lys587
Accession # Q0PHV7
Pro486-Lys587
Accession # Q0PHV7
Specificity
Detects human and mouse DACT3/Dapper 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DACT2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse DACT3/Dapper 3 Antibody
Detection of Human and Mouse DACT3/Dapper 3 by Western Blot.
Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse DACT3/Dapper 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for DACT3/Dapper 3 at approximately 45 kDa with human brain and 50 kDa with mouse brain (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human/Mouse DACT3/Dapper 3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human brain tissue and mouse brain tissue
Sample: Human brain tissue and mouse brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DACT3/Dapper 3
Dapper 3 (also Dact 3) is a 63 kDa (predicted) member of the DACT (Dapper/Frodo) family of Dvl-binding proteins. It is expressed in cerebral cortex and uterus where it antagonizes Wnt/ beta-catenin signaling. When present in colorectal tumor cells, it initiates apoptosis. Both effects are likely the result of a Dapper 3 interaction with Dvl2, thereby blocking beta-catenin entry into the nucleus. Mouse Dapper 3 is 610 amino acids (aa) in length It contains one coiled-coil region (aa 63-87), an Arg-rich domain (aa 351-476), and a PZD-binding motif (aa 607-610). There is one potential splice variant that shows a 45 aa substitution for aa 413-610. In human Dact 3 the frameshift at position 119 is reported to yield a protein product with estimated moleqular weight of 41 kDa. Over aa 486-587, mouse dapper 3 shares 82% and 99% aa identity with human and rat dapper 3, respectively.
.
Long Name
Dapper, Antagonist of beta-Catenin, Homolog 3
Alternate Names
Dapper 3, RRR1
Gene Symbol
DACT3
UniProt
Additional DACT3/Dapper 3 Products
Product Documents for Human/Mouse DACT3/Dapper 3 Antibody
Product Specific Notices for Human/Mouse DACT3/Dapper 3 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...