Human/Mouse DDX5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6370
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 632813
Product Specifications
Immunogen
E. coli-derived recombinant human DDX5
Asn448-Gln614
Accession # P17844
Asn448-Gln614
Accession # P17844
Specificity
Detects human and mouse DDX5 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse DDX5 Antibody
Detection of Human and Mouse DDX5 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse DDX5 Monoclonal Antibody (Catalog # MAB6370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DDX5 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse DDX5 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB6370 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DDX5
DDX5, also known as p68 RNA helicase, is a 614 amino acid (aa), 68 kDa member of the DEAD box family of RNA helicases that contain the conserved Asp‑Glu‑Ala‑Asp (DEAD) motif. DDX5 influences RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. DDX5 shares 87% aa identity with DDX17 within the central region and the two are often expressed together. The C-terminal region used as an immunogen shares 96% and 99% aa identity with mouse and rat DDX5, respectively, but lies mainly outside the DDX17 homology region.
Long Name
DEAD Box Protein 5
Alternate Names
DEAD/H, G17P1, HELR, HLR1, HUMP68
Gene Symbol
DDX5
UniProt
Additional DDX5 Products
Product Documents for Human/Mouse DDX5 Antibody
Product Specific Notices for Human/Mouse DDX5 Antibody
For research use only
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