Human/Mouse ELF3 Antibody

Detection of Human ELF3/ESE-1 by Western Blot

Inhibition of the SE-associated key targets attenuated LUAD malignant progression via the suppression of master TFs in CRC model.A–C Perturbation of SE associated key targets, including BRD4, EP300, and CDK7, by small-molecule inhibitors reduced the expression of master TFs significantly at protein levels. D Expressions of master TFs in LUAD cell line PC-9 treated with SE associated targets inhibitors in mRNA level were analyzed by real-time PCR. E Inhibition of invasive and migrate activity by perturbation of SE associated key targets via small molecular inhibitions. PC-9 cells were cultured with indicated small molecular inhibitions and subjected to invasion and migration assays (see “Materials and methods”). Invaded and migrated cells were stained with crystal violet and counted. Representative photographs were shown. F, G Histograms represent the number of invasion or migration cells. H In PC-9 cell lines, alterations of ELF3, EHF, and TGIF1 at protein levels while JQ1 treated for 100 nM 48 h together with or without transfected with overexpression plasmids. GAPDH and beta-actin were used as internal control, the lower panels showed the gray scale ratio of protein (ELF3) to GAPDH and protein (EHF and TGIF1) to beta-actin. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33070167), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human ELF3/ESE-1 by Western Blot

Inhibition of the SE-associated key targets attenuated LUAD malignant progression via the suppression of master TFs in CRC model.A–C Perturbation of SE associated key targets, including BRD4, EP300, and CDK7, by small-molecule inhibitors reduced the expression of master TFs significantly at protein levels. D Expressions of master TFs in LUAD cell line PC-9 treated with SE associated targets inhibitors in mRNA level were analyzed by real-time PCR. E Inhibition of invasive and migrate activity by perturbation of SE associated key targets via small molecular inhibitions. PC-9 cells were cultured with indicated small molecular inhibitions and subjected to invasion and migration assays (see “Materials and methods”). Invaded and migrated cells were stained with crystal violet and counted. Representative photographs were shown. F, G Histograms represent the number of invasion or migration cells. H In PC-9 cell lines, alterations of ELF3, EHF, and TGIF1 at protein levels while JQ1 treated for 100 nM 48 h together with or without transfected with overexpression plasmids. GAPDH and beta-actin were used as internal control, the lower panels showed the gray scale ratio of protein (ELF3) to GAPDH and protein (EHF and TGIF1) to beta-actin. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33070167), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human ELF3/ESE-1 by Western Blot

The regulatory interaction network in CRC model of LUAD-SE-associated TFs is interdependent.A–C, J Expressions of all master TFs in the knockdown of any master TF and validating the efficiency of siRNA targeting to master TFs by both real-time PCR and western blot. D–I Western blot and real-time PCR demonstrating that siRNA co-transfected with pcDNA3.1 could decrease the master TF expression significantly in both protein and mRNA level. This phenomenon could be partly reversed by either of another two master TFs, also could be reversed almost entirely in the combination of another two master TFs. K–M Data are shown as fold enrichments of master TFs promotor sub-regions in each antibody immunoprecipitate, ELF3 antibody (K), EHF antibody (L), TGIF1 antibody (M), over control IgG immunoprecipitate. GAPDH and beta-actin were used as internal control, the lower panels showed the gray scale ratio of protein (ELF3) to GAPDH and protein (EHF and TGIF1) to beta-actin. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33070167), licensed under a CC-BY license. Not internally tested by R&D Systems.