Human/Mouse FosB/G0S3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2214
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 269928
Product Specifications
Immunogen
E. coli-derived recombinant human FosB
Met1-Leu338
Accession # P53539
Met1-Leu338
Accession # P53539
Specificity
Detects human and mouse FosB in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse FosB/G0S3 Antibody
Detection of Human and Mouse FosB/G0S3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with TPA for 2 hours and NIH-3T3 mouse embryonic fibroblast cell line untreated or treated with serum for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse FosB/G0S3 Monoclonal Antibody (Catalog # MAB2214), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FosB/G0S3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse FosB/G0S3 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: PMA-treated HeLa human cervical epithelial carcinoma cell line and serum-treated NIH-3T3 mouse embryonic fibroblast cell line
Sample: PMA-treated HeLa human cervical epithelial carcinoma cell line and serum-treated NIH-3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FosB/G0S3
FosB (FBJ murine osteoscarcoma viral oncogene homolog B) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. AP-1 activity is important for gene transcription in a wide variety of cellular processes.
Long Name
FBJ Murine Osteosarcoma Viral Oncogene Homolog B
Alternate Names
G0S3
Gene Symbol
FOSB
UniProt
Additional FosB/G0S3 Products
Product Documents for Human/Mouse FosB/G0S3 Antibody
Product Specific Notices for Human/Mouse FosB/G0S3 Antibody
For research use only
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