Human/Mouse HAND2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3876
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HAND2
Met1-Gln217
Accession # P61296
Met1-Gln217
Accession # P61296
Specificity
Detects human and mouse HAND2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human HAND1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse HAND2 Antibody
Detection of HAND2 in Mouse Embryo.
HAND2 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Goat Anti-Human/Mouse HAND2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3876) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in developing tongue. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse HAND2 Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo
Sample: Immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo
Western Blot
0.1 µg/mL
Sample: Recombinant Human and Mouse HAND2
Sample: Recombinant Human and Mouse HAND2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF3876 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAND2
Long Name
Heart And Neural Crest Derivatives Expressed 2
Alternate Names
dHand, Ehand2, Hed, Th2, Thing2
Gene Symbol
HAND2
UniProt
Additional HAND2 Products
Product Documents for Human/Mouse HAND2 Antibody
Product Specific Notices for Human/Mouse HAND2 Antibody
For research use only
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