Human/Mouse ING1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5758
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 585915
Product Specifications
Immunogen
E. coli-derived recombinant human ING1
Arg70-Ala184
Accession # Q9UK53
Arg70-Ala184
Accession # Q9UK53
Specificity
Detects human and mouse ING1 in Western blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse ING1 Antibody
Detection of Human and Mouse ING1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HT-2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ING1 Monoclonal Antibody (Catalog # MAB5758) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ING1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse ING1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and HT‑2 mouse T cell line
Sample: HepG2 human hepatocellular carcinoma cell line and HT‑2 mouse T cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ING1
ING1 (inhibitor of growth-1) is a member of the ING family of tumor suppressive and apoptosis-promoting epigenetic regulators of chromatin structure. They interact with and alter the ratio of pro- and anti-apoptotic Bcl-2 family members. The p53 tumor suppressor and its downstream targets can potentiate ING1-induced apoptosis. Human isoforms p33ING1, p24ING1c, p33ING1b and p47ING1a vary in N-terminal sequence, resulting in 279, 210, 235 and 422 aa proteins, respectively. The sequence used as an immunogen is common to all isoforms, and shares 83% and 81% amino acid (aa) identity with mouse and rat ING1, respectively.
Long Name
Inhibitor of Growth 1
Alternate Names
p24ING1c, p33ING1b, p47ING1a
Gene Symbol
ING1
UniProt
Additional ING1 Products
Product Documents for Human/Mouse ING1 Antibody
Product Specific Notices for Human/Mouse ING1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...