Human/Mouse LRRN1/NLRR-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4990
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Xenograft
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRRN1
Ser26-Ala631
Accession # AAH34947
Ser26-Ala631
Accession # AAH34947
Specificity
Detects human LRRN1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NLRR-3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse LRRN1/NLRR-1 Antibody
LRRN1/NLRR‑1 in Embryonic Mouse Somites.
LRRN1/NLRR-1 was detected in immersion fixed frozen sections of embryonic mouse somites (E9.5) using 10 µg/mL Sheep Anti-Human LRRN1/NLRR-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4990) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse LRRN1/NLRR-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse somites (E9.5)
Sample: Immersion fixed frozen sections of embryonic mouse somites (E9.5)
Western Blot
0.1 µg/mL
Sample: Recombinant Human LRRN1/NLRR-1
Sample: Recombinant Human LRRN1/NLRR-1
Reviewed Applications
Read 1 review rated 5 using AF4990 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRN1/NLRR-1
LRRN1 (Leucine-rich repeat neuronal protein 1; NLRR1) is a 95 kDa member of the neuronal LRR family of proteins. It is a cell-surface glycoprotein that is expressed on embryonic motor neurons and somatic myoblasts. Human LRRN1 is 716 amino acids (aa) in length. It is a type I transmembrane protein that contains a 606 aa extracellular region (aa 26-631) and a short 64 aa cytoplasmic tail. The extracellular region shows eleven LRRs (aa 94-384) that mediate protein-protein interactions, one C2-type Ig-like domain and one fibronectin type III domain. There is one potential alternate start site at Met286. Over aa 26-631, human LRRN1 shares 96% aa sequence identity with mouse LRRN1.
Long Name
Leucine-rich Repeat Neuronal 1
Alternate Names
FIGLER3, LRN, NLRR-1, NLRR1
Gene Symbol
LRRN1
UniProt
Additional LRRN1/NLRR-1 Products
Product Documents for Human/Mouse LRRN1/NLRR-1 Antibody
Product Specific Notices for Human/Mouse LRRN1/NLRR-1 Antibody
For research use only
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