Human/Mouse MAGI2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7117
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MAGI2
Ser2-Arg130
Accession # Q86UL8
Ser2-Arg130
Accession # Q86UL8
Specificity
Detects human MAGI2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant ARIP-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse MAGI2 Antibody
Detection of Human MAGI2 by Western Blot.
Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MAGI2 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MAGI2 in U‑87 MG Human Cell Line.
MAGI2 was detected in immersion fixed U-87 MG human glioblastoma/ astrocytoma cell line using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MAGI2 in Human Brain.
MAGI2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons and neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse MAGI2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line
Sample: Immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Western Blot
1 µg/mL
Sample: Human brain (cortex) tissue
Sample: Human brain (cortex) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAGI2
MAGI2 (AIP-1, ACVRINP1 also known as Activin receptor-interacting protein 1 and S-SCAM in rodents); is a 160‑180 kDa member of the MAGUK family of proteins. It is found in neuronal post-synaptic membrane complexes, and serves as a molecular scaffold for multiple proteins, including alpha-actinin, dendrin, SMAD3 and beta‑catenin. ARIP-1 facilitates the signaling of both growth factor and neurotransmitter receptors such as ActRIIA, NMDA and beta1-adrenergic receptors. Human ARIP-1 is 1455 amino acids (aa) in length. It contains an N-terminal PZD domain (aa 17-101), followed by a guanylate kinase-like domain (aa 109-283), two WW domains (aa 302‑381) and five subsequent PZD domains (aa 426-1229). ARIP-1 is reported to dimerize/oligomerize. There are three potential isoform variants. All utilize an alternative start site at Met164 that may be accompanied by either an Arg substitution for aa 757-771, or a 48 aa substitution for aa 1236-1455. Over aa 2-130, human and mouse ARIP-1 are identical in aa sequence.
Long Name
Membrane-associate Guanylate Kinase Inverted 2
Alternate Names
ACVRINP1, ACVRIP1, AIP1, ARIP1, SSCAM
Gene Symbol
MAGI2
UniProt
Additional MAGI2 Products
Product Documents for Human/Mouse MAGI2 Antibody
Product Specific Notices for Human/Mouse MAGI2 Antibody
For research use only
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